Extraction of Recombinant Dog Gastric Lipase from Transgenic Corn Seed
نویسندگان
چکیده
منابع مشابه
Extraction of recombinant dog gastric lipase from transgenic corn seed.
Several approaches were examined for extracting the relatively hydrophobic protein recombinant dog gastric lipase (rDGL) expressed in the endosperm of transgenic corn seed. The first approach used minimal processing of the seed before extraction (i.e. simple grinding of whole seed) followed by selective extraction to eliminate 72% of contaminant proteins without compromising rDGL recovery from ...
متن کاملEnzymatic assay method for evaluating the lipase activity in complex extracts from transgenic corn seed.
A colorimetric method was established to determine the activity of recombinant lipase in extracts from transgenic corn seed. The system was an oil-in-water emulsion that was stabilized by a surfactant to accommodate the organic phase substrate and aqueous phase enzyme. The lipase activity was measured by monitoring the release of nitrophenol at 346 nm from the substrate, 4-nitrophenyl butyrate....
متن کاملRecombinant Human Proinsulin from Transgenic Corn Endosperm: Solvent Screening and Extraction Studies
Recombinant pharmaceutical proteins are being produced in different systems such as bacteria and mammalian cell cultures. The use of transgenic plants as bioreactors has recently arisen as an alternative system offering many practical and economic advantages. However, finding an optimum strategy for the downstream processing (DSP) of recombinant proteins from plants still remains a challenge. I...
متن کاملOil Extraction from Millet Seed – Chemical Evaluation of Extracted Oil
ABSTRACT: The object of this study is to extract the oil from millets and evaluate the chemical properties of the extracted oil. Commercial millet oil was obtained by cold solvent extraction adopting soxhlet procedure. Quantitative and qualitative tests concerned with the non-saponifiable matter, fatty acid composition, phosphorus content representing total phospholipids, refractive index, pero...
متن کاملextraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Agricultural and Food Chemistry
سال: 2006
ISSN: 0021-8561,1520-5118
DOI: 10.1021/jf061921h